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ASCP ASCP-MLT Exam Syllabus Topics:

Section	Weight	Objectives
Microbiology	15–20%	- Bacteriology and mycology procedures - Virology and parasitology testing
Urinalysis and Other Body Fluids	5–10%	- Microscopic analysis and disease correlation - Physical and chemical testing
Immunology	5–10%	- Serologic procedures and immune diseases - Principles of immunology
Laboratory Operations	5–10%	- Quality assessment and safety - Instrumentation and laboratory mathematics
Hematology	20–25%	- Laboratory testing and hemostasis - Physiology and disease states
Blood Banking	15–20%	- Blood products and components - Blood group systems and immunology - Transfusion practice and testing
Chemistry	20–25%	- Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. No HLA matching is performed for corneal tissue transplants as the cornea occupies a privileged site not usually seen by the immune system. This term has been coined "immune privileged".
Corneal tissue may be transplanted successfully from one patient to another because:

A) Corneal antigens do not activate T cells
B) The cornea has the same DNA in everyone.
C) The cornea is non-antigenic
D) Anti-corneal antibodies are easily suppressed
E) The cornea occupies a privileged site not usually seen by the immune system

2. An ultrarapid metabolizer (UM) would require a higher dose of a drug than an EM (a person with normal enzyme activity) since the UM eliminates the drug more quickly.
A CYP2D6 ultrarapid metabolizer (UM) would require ___________ dose of an active drug (non-prodrug) that is metabolized by CYP2D6 than a CYP2D6 extensive metabolizer (EM).

A) the same
B) a higher
C) a lower

3. Thyroid-stimulating immunoglobulins, or TSI's, are IgG antibodies that can bind to thyrotropin (TSH) receptors on the thyroid gland. TSIs mimic the action of TSH, causing excess secretion of thyroxine and triiodothyronine. The TSI level is abnormally high in persons with hyperthyroidism due to Graves' disease.
Which of the following is an autoantibody that binds to TSH receptor sites on thyroid cell membranes preventing thyroid-stimulating hormone binding?

A) Antimicrosomal antibodies
B) Antithyroglobulin
C) Thyroid-stimulating immunoglobulins
D) Lupus erythematosus
E) Thyroxin-binding globulins

4. The ratio of blood to anticoagulant in a light blue stopper tube is:

A) 10:1
B) 9:1
C) 2:1
D) 5:1

5. The correct answer is Trichophyton rubrum. The tiny microconidia are lined up in a "birds on the fence" arrangement along the hyphal strand transversing the field of view (yellow arrows). Two pencil-shaped, smooth walled macroconidia are also seen in the lower left field of view (red arrows).
Trichophyton verrucosum may produce microconidia in small quantities. However, they are irregularly arranged with little tendency to line up along the hyphae. Antler hyphae and string bean macroconidia are characteristic of this species.
One of the key characteristics in the identification of Epidermophyton floccosum is the inability of this dermatophyte to produce microconidia. Two to four-celled, club-shaped macroconidia are produced, usually in clusters of two or three.

The profusion of tiny microconidia lining up along the hyphae in this photomicrograph is characteristic of which of the following organisms?