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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is using a nucleic acid amplification test (NAAT) to detect Mycobacterium tuberculosis directly from sputum. A positive result indicates:

A) The presence of viable M. tuberculosis organisms.
B) Active tuberculosis disease requiring immediate treatment.
C) Drug susceptibility information for the M. tuberculosis strain.
D) The presence of M. tuberculosis DNA, which could be from viable or non-viable organisms.

2. A patient with AIDS develops a disseminated fungal infection. Blood cultures grow a yeast that produces pseudohyphae and terminal chlamydospores on cornmeal agar. The patient's CD4+ T-cell count is below 50 cells/µL. The MOST likely causative organism is:

A) Candida albicans
B) Cryptococcus neoformans
C) Aspergillus fumigatus
D) Pneumocystis jirovecii

3. A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25°C. Which organism is most likely?

A) Corynebacterium jeikeium
B) Bacillus anthracis
C) Listeria monocytogenes
D) Erysipelothrix rhusiopathiae

4. An acid-fast bacillus isolate recovered from a respiratory specimen after 3 weeks of incubation on Löwenstein- Jensen medium is initially non-pigmented when grown in the dark. After exposure to light for several hours, the colonies develop a distinct yellow-orange pigment. This organism is classified as a:

A) Nonchromogen
B) Rapid grower
C) Scotochromogen
D) Photochromogen

5. A patient with a history of inflammatory bowel disease is diagnosed with primary sclerosing cholangitis and undergoes liver transplantation. Post-transplant, they develop a bloodstream infection with a Gram-negative bacterium that is identified as Fusobacterium nucleatum. This organism's role in the pathogenesis of colorectal cancer is primarily attributed to its ability to:

A) Form biofilms that physically obstruct the bile ducts.
B) Directly induce mutations in colonic epithelial cells.
C) Modulate the host immune response and promote a pro-inflammatory microenvironment.
D) Produce potent exotoxins that damage the intestinal epithelium.

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